I can’t believe the fifth week of the MARC-SPUR summer program is almost over! Currently busy studying for the GRE and taking the Princeton course offered by the SPUR program which has been a huge help. The latest workshops on how to apply for graduate level funding have been very insightful in showing other potential funding sources besides graduate level institutions. I have also started filling out secondary applications to several M.D.-Ph.D. programs and hope to send them out very soon.
In terms of research, I have been busy studying the Rbfox proteins which are known tissue-specific splicing regulatory proteins. So far I have purified several Rbfox protein constructs which have deletions in the N- and C-termini to see if these mutations may affect RNA binding. I have also been trying to purify other proteins such as heterogeneous ribonucleoprotein F (hnRNP F) which is known to regulate splicing and mRNA stability and the KH type splicing regulatory protein (KSRP). I hope to simultaneously assay these proteins with an RNA substrate to see if there are possible protein-protein interactions that may affect protein-RNA binding.
Performing in vitro transcription to synthesize the radio-labeled RNA substrate.
Bacterial culture used for hnRNP F protein expression.
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