So I attempted cloning
again to try and get an insert for 2 of my mutants and the wildtype RRM2,
hoping that the third time was the charm. However after the letdown of Cloning
attempts 1 and 2 (Cloning attempt 1= only 1 out of 3 of the inserts in bacteria;
Cloning attempt 2= no inserts/bacteria!), I packed my bag for the beach this
morning, with the intention of checking to see if I had bacterial colonies from
my cloning and if there were none (which was the case the last time) I was
going to do my labwork (work on assignments due for the MARC program as well as
reading scientific papers) on the beach. Win-win situation, right? I thought
so. So I walk into lab, and then to the common equipment room where I let my
bacterial plates incubate overnight, telling myself that if I didn’t get
colonies this time, it’s okay, I’ll get them next time and at least I get to
enjoy the beach…and Wah-Lah!!! I had colonies on all of my plates, and none on
the ligation control plate, which is EXACTLY what I was hoping for!!! (here is
a very fuzzy picture of my plates haha…basically I got bacterial colonies and
could proceed to the next step!....New phone with a better camera coming soon!
lol)
So I excitedly texted
BJ, my mentor, (oh I didn’t show you a picture of us huh? Here you go haha)
Anyway, I excitedly texted him saying I got colonies, and he
gave me the okay to go to the next step (he was on his way to lab). So today,
I’ve been busy picking colonies, doing colony PCR, and I just finished loading
my gel…We’ll see if the colonies I picked have an insert! I really hope so,
because if they do it’s on to a miniprep and then sequencing to REALLY confirm
that I did my cloning correctly; wish me luck! :D
That’s what I was looking for guys!!! ^ (not the circle; the
bright bands). I’m so excited! Progress is being made! :DThanks for sharing my excitement :) I’ll write again soon!
-Tay
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