Friday, August 29, 2014

Nancy Morones Summer 2014 Blog Post 1


 Hello! My name is Nancy Morones and I am currently involved in the UCLA Summer Programs for Undergraduate Research (SPUR). I have been busy at work in Dr. Karen Lyons laboratory—under the direction of Dr. Jay Jiang—within the department of Orthopaedics.

My project focuses on the CCN family of matricellular proteins and their effect on articular cartilage and maintenance. These modular proteins have been associated with having high integrin binding activity and have an essential role in cell migration, adhesion and proliferation.  In the case of chondrocytes, cells that form cartilage and bone, CCN proteins such as Cys61 (CCN1) and Connective Tissue Growth Factor (CCN2, CTGF) have been found to play a role in cell differentiation in early stages of development for mice models. I plan to study the localization of gene expression in CCN1 and CCN2 mice models at several ages using immunohistochemistry and immunofluorescence.

Being in the SPUR program has been very beneficial to my future as a scientist. The program has several weekly workshops that have informed me about the graduate school application process as well as MSTP programs. I really enjoyed the panels run both by students and by professors. As part of the Society for Advancement of Chicanos/Latinos and Native Americans in Science (SACNAS) Chapter here at UCLA, I was also able to present a bit about my undergraduate research experience at an event hosted by the Outreach Committee for Diversity in Science (OCDC) to community college students. As a transfer student, was both an honor and privilege to share my experiences with other students with similar goals. 



Thursday, August 28, 2014

Benni Vargas, Summer 2014, Blog Post 1

Hello everyone this is Benni Vargas and I am a 4th year MIMG student.  I am currently doing summer research under the CARE SEM Summer Research Program. I recently got into MARC and I am doing research in Dr. Robert L. Modlin’s lab in BSRB in room 246.

Using leprosy as a model, our research is concerned with the responses stimulated in B cells as measured by cellular proliferation in tuberculoid leprosy versus lepromatous leprosy. We hope to gain insight into the role B cells play in the immune response to Mycobacterium leprae, an intracellular pathogen that causes leprosy. This information may provide new possibilities for advancement of treatments for various human diseases.

We have had numerous attempts in creating a positive control for B cell stimulation. We need a positive control in order to confirm that indeed B cells are being stimulated in the presence of M. leprae. To do this, we have isolated peripheral blood mononuclear cells (PBMCs) from blood, isolated B cells from those PBMCs, and plated the B cells with various stimulatory reagents in hopes of getting them to proliferate.

Hopefully soon after we establish our positive control we can actually do some experiments with M. leprae!

It has been quite an experience so far here at the Modlin lab. At first I was working very dependently with my direct mentor. Now, I am becoming more independent. In fact I am doing an experiment by myself tomorrow! My direct mentor is gone for clinical duties tomorrow and it is up to me to carry out this experiment. This will be fun.


In the picture below I am isolating PBMCs from blood. The PBMCs are at the interphase between the ficoll (colorless) layer and the plasma (orange) layer:


In the picture below I have plated my B cells with various concentrations and combinations of stimulatory reagents such as immunoglobulin H + L, CD40 antibody, and immunoglobulin M antibody. Sorry for the picture quality (I used flash)!



Tien Phan, Summer 2014, Blog Post 1

Hello!

I guess I should introduce myself in the first blog. My name is Tiên. I am a senior majoring in Molecular, Cell, and Developmental Biology and minoring in Biomedical Research (and possibly Bioinformatics). I am currently working in Dr. Karen Lyons's lab under the department of orthopedic surgery. My research project revolves about the proteins CCN1 and CCN2. Since not a whole lot is known about them, we are investigating their roles in embryonic cartilage differentiation, the molecular pathways that they're involved in, and the relationship between them.

I am now trying to finish the BrdU incorporation study which uses fluorescence immunohistochemistry to look at cartilage cell proliferation in mouse embryos. I am also currently responsible for the primary mesenchymal cell pellet study which looks at the effect of CCN1 and CCN2 on osteogenesis differentiation. 


I have been learning so much since I joined Dr. Lyons lab. I am constantly learning new skills, and I am absolutely loving it! I learned cell culture, immunostaining, animal handling... I get to work with mice, mouse embryos, viruses... Our lab uses the mouse model, so I got to learn mouse dissection which never fails to give me the thrills! Everyday in lab, I feel like a sponge, and I am super eager to absorb new knowledge that comes my way. I am so grateful to have an awesome mentor who's patiently taught me so much, who challenges me with projects while always giving me the needed support and guidance. Below is a group picture we took last quarter, though not everyone was in it. I am the fourth person from the left, wearing a white lab coat.


I really like it here. So much that I've decided to nest. I have my gallon of milk, my cereal, noodles, and my cup in the lunch room. I also have Lilian and Amelia hanging out on the window sill next to my desk.


 I can't wait to see what the next 2 years have in store :D

Peace out!


Rachel Lopez, Summer 2014, Blog Post 1

Research this summer, along with the seminars and workshops I’ve been attending for SPUR, has been quite the experience. I have been give information through the seminars and workshops that will prove to be useful for my career such as writing for and looking for grants to help fund my education. In lab, I have been learning a lot of new techniques as well as mastering the techniques that I have acquired over the past year in my lab. This week started with isolating hearts for my project. Here is a picture of me holding a tube with three separate layers. It’s a step in the protocol that I have come to have a love hate relationship with because it’s been difficult to get just right. The top layer is all the organelles from the cells that are not what I need for my extraction. The middle is the buffer that separates everything in nucleus, which is found at the bottom of the tube. This next picture is of the standard curve for my quantification of the amount of proteins I’ve isolated from the whole hearts. This is what a standard week looks for me in lab.



Walter Hardesty, Summer 2014, Blog Post 1

Hi MARCers!

I am checking in after an entire year of my rollercoaster-ride of a life at UCLA…including my summer at Harvard Medical School! This past summer, I spent 10-weeks in Boston, MA,  participating in a summer program called the Harvard Catalyst Summer Clinical and Translational Research Program (SCTRP).


Here’s me before my flight:


I must admit, I was EXCITED to get to Boston. Little did I know of the challenges (though rewarding!) that were in store for me…
AT BOSTON, I worked under the guidance of a Harvard Faculty mentor, Dr. Gabriel Kreiman, and my specific project investigated the contribution of recurrent processing in the visual system to partial object recognition.

As part of my project, I set out to recruit over 70 participants from the Harvard Medical School Area to complete various forms of a simple object recognition experiment. The visual test, coded by my graduate student mentor, Hanlin Tang (a physics major from Princeton University who worked for the US Department of Defense), consisted of displaying 16 different images that pertained to one of various categories. Subjects were asked to categorize images that varied in their length of time displayed, object wholeness (i.e. whole or partial), and whether they were followed by a backward mask or not. The backward mask is a noisy image that follows the initial presentation of an object stimulus. It is believed in theories of natural vision that by following an image with a backward mask, one is able to disturb recurrent feedback in the brain, thereby decreasing categorization performance.

After collecting data, I used MatLab, a computer prograaming software, to perform data analysis on the response data gathered from our wonderful subjects in order to address the following question:

Is top-down processing vital for categorization performance of partial images irrespective of how features are positioned?

Can a model be developed to make predictions on which features of an image a) drive categorization performance and b) require top-down processing neural networks?

            I won’t get into too much detail here, but I must say, the data analysis was EXTREMELY difficult at first. Before arriving to my lab at Boston Children’s Hospital, I had little to no experience with computer science in general. My major is in microbiology, immunology, and molecular genetics. I was used to pipetting, and cloning genes to make different parasitic cell lines . On the first day of my new lab, I sat in front of a computer and feared that I would be incapable of getting up-to-speed with the work in the lab in order to produce something significant. However, I pushed forward, and I was reminded of the valuable lessons in research methodology that I gained from the MARC Program. MARC taught me how to ask the right questions, and how to structure the knowledge in a way that made sense. I learned how to use Matlab! And I learned how to code! Though, I surely was not alone in these efforts. Hanlin Tang was hugely instrumental to my learning process. He was patient with all of my questions, and pushed me to succeed in the lab. In addition, Candace Ross, a computer engineering major from Howard University who was also interning for the summer through MIT, provided me lessons in basic computer science that SAVED me during difficult times in the analysis. I am truly grateful for everyone’s contribution from the lab, including my PI and other lab members.

Here are snapshots of me, Hanlin, and two “computer-wiz’ highschoolers (center left and center right) interning for the summer as well:


Besides my lab, the Harvard SCTRP Program also consisted of various presentations, tours, and outings with my fellow peers from the program.  I am deeply grateful for Ms. Carol Martin, Program Manger, and Mrs. Rachel Milliron, Program Coordinator, both of whom have opened doors for us to network and LEARN from some of the top scientists and educators at Harvard into our doors.  Because of them, I have acquired a new meaning for the word professionalism, which was a way of life for us in the Harvard SCTRP Program, and will continue to be a way of life for me now in my academic career.

In addition, I met students from all across the nation, including Jackson, Missippi; New Orleans, Louisiana; Las Vegas, Nevada to name a few. One of the things I’m most grateful for from this program is the awareness that I gained from living with students who were of different cultures than me, and understanding that we each had a unique approach to pursue a common goal for a career in academic medicine. Some of these students are the most inspiring I have ever met. I’d like to give a shoutout to one individual, Kia Byrd, a rising first-year Harvard Medical Student, who has inspired me to reach for the stars! Below is a picture of all of us in front of the Harvard Medical School sign!


While living for roughly 70 days in Boston, Massachusetts, I also visit a huge number of places at Boston, and even New York during a weekend get-a-away with one of my peers. This was my first time on the East Coast of the country. During my stay, I visited Harvard University (including most of Harvard Square), Cambridge, MIT, Boston Harbor, the North End, Little Italy, Little Colombia (near the New Hampshire passway), and various hospital and research sites around the Harvard Medical School Area. Dr. Dwayne Simmons even treated me out to dinner, and gave me a more thorough tour of Harvard University, including the freshmen dorms! The North End was one of my favorite places due to sheer volume of historical sites. Here I visited Paul Revere’s House, the graveyards of many war heroes and founding fathers, the Old North Church, the Freedom Trail, Haymarket, the Constitution (oldest, warship still floating on water) etc. (THE LIST GOES ON) At these locations, I learned of an entirely new culture that was marked by historical reverence, intellectualness and an air of prestige. I am proud to say that I have done the Duck Tour TWICE. AND I have tried Boston lobster…with my mother even when she came to visit! Look how delicious:


And yes! That’s right! My mother came to visit me, and it was HER first time on the East Coast too. Check us out on the carriage for proof!


In New York, I saw the Statue of Liberty, the World Trade Center memorials, the renovated twin towersm the Bull of Wall St., memorials for the Vietnam and Korean Wars, Central park, Time Square, the Brookline Bridge, the skyline of New Jersey

The experience of travelling on the East Coast this past summer is one I’ll never forgot. After returning to Los Angeles, California, I found that my life has changed. I work harder than ever before, which is reminiscient of the extended efforts I observed in the professions of those I worked closely with at Boston Children’s Hospital and Harvard Medical School. Also, I am more conscientious of the history that lies behind the places that I visit now, including those in Los Angeles now! For all of those reading this blog, and have yet to spend a summer outside of UCLA, I would HIGHLY recommend it. The experience will change you in ways that are not limited to what you learn in the lab. Your vision of the world, of the various institutions you visit, of people, and of yourself will change in ways that you will be glad they did!





Steve Guzman, Summer 2014, Blog Post 1

Blog Submission #1:


Greetings from Los Angeles!  I’m Steve Guzman and this is my final summer research experience here at UCLA.  Currently, I am completing a first authorship manuscript.  These past few weeks have been extremely busy trying to wrap up my project.  I am writing an article about the longitudinal changes that occur within single motor units in upper limb rhesus monkeys after a spinal injury.  I spend most of my days generating figures and reading articles.  At times it can be quite tedious work but my main motivation is that I’ll have my first publication out soon! Aside from working on my project I’ve been mentoring new incoming transfer students who are participating in summer research programs.  Aside from all the work at lab and the mentoring, I’ve been playing a lot of beach volleyball on the weekends.  I’ve realized that this could possibly be my last summer in Los Angeles or even California for a while, and so I must enjoy the beach as much as possible! The first image is a picture of me (don’t mind my goofy expression) at Bruin Plaza when UCLA was flooded by the main water line.  Thankfully, no one was harmed. The second picture is of me at Will Rogers Beach playing beach volleyball.  I hope all my other fellow MARCers are enjoying themselves this summer and I can’t wait to hear from them soon!



Mark Douglass, Summer 2014, Blog Post 1


Hello everyone! I hope you are all having a wonderful summer. My name is Mark Douglass and this summer I am working in Dr. Ren Sun’s lab in the Molecular and Medical Pharmacology Department at the UCLA David Geffen School of Medicine. My direct mentor is associate professor Dr. Ting-Ting Wu, and all of our group’s projects focus on the molecular virology of gammaherpes viruses. I have been working in this lab since March, and things are really going well now that I get to dedicate so much time here this summer. My specific project focuses on understanding the molecular function of open reading frame 73 (ORF73), a specific protein expressed during the latent stage of infection. This is important because the latent stage of infection is linked to oncogenesis. We use Murine Herpes Virus 68 as a model for the human pathogens Epstein-Barr virus and Karposi’s Sarcoma-Associated Herpes virus.
I have been using basic methods such as the polymerase chain reaction, gel electrophoresis, cloning, and cell culture to develop a recombinant virus; which will allow me to study the molecular interactions of our gene of interest using co-immunoprecipitation and mass spectrometry. The overarching goal is to identify possible target mechanisms and molecules for antiviral treatments.
The weeks I’ve dedicated thus far to the lab, and the MARC program, have been very rewarding. I am beginning to comprehend what it’s truly like to be a scientist. The workshops and seminars that we are supplemented with have been invaluable; they have offered guidance and brought awareness to the possibilities within the STEM field. I have also enjoyed living in the dorms, meeting people from all around the country, and going on group trips to the Getty and the Griffith Observatory.




Taylor Brown, Summer 2014, Blog Post 5

Blog 5:


Hey guys! I have one more surprise blog for you! I took a spontaneous trip to NY my last weekend on the East Coast!!! Here are pictures; it was my first time in NY!!! Big shout out to my high school cross country brother Edwin (Enrique, as I call him) Melon for agreeing to go with me!!! It was so much fun!


(me and Edwin, our first night in the city!)


(on a boat on our way to Lady Liberty)





(Times Square is so BRIGHT at night! Haha)



Remembering 911: Twin Tower Memorial fountain number 1


Remembering 911: Twin Tower Memorial fountain number 2



Remembering 911: The Freedom Tower
(we also went into the 911 museum as well; it was HUGE)


My favorite part; Central Park! <3 o:p="">


(the hot dogs we bought were massive! Haha)


Thanks for reading guys! Take care!
-Tay<3 o:p="">













Taylor Brown, Summer 2014, Blog Post 4

Hey Guys!

So I decided to write one more [short] blog, afterall haha. This one has pictures from my Poster Session and our last MSRP (MIT Summer Research Program) outing!. Both were tons of fun(:


The group at our Poster Session(:


Just us Girls


The HHMI EXROP girls (Lindsey and Rana) with each of our posters behind us.


My mentor Thales Papagiannakopoulos and I in front of my poster(:
[his last name is awesome, right?!]


Dr. Tyler Jacks and I! :D
And below are a couple of random pictures from our last MSRP outing


Sheena and Vanessa


Li, Me, Pablo, Rana, Lindsey, and Nadege :D


Playing in a Boston Fountain(:


We really bonded, what can I say? Haha. Largest lap-sit I’ve ever been in haha


Scientists in the Willows


And although, I haven't hit rock bottom, there's still nowhere to go but up ! :D
Time to reach for the moon (like my sister’s recently published children’s book titled Imani’s Moon : https://www.facebook.com/imanismoon?fref=ts ; http://www.amazon.com/Imanis-Moon-Janay-Brown-Wood/dp/1934133582 ) and apply to grad school to follow my dream of becoming a cancer researcher! Wish me luck everyone, and thank you for reading!

Sincerely,
Taylor Brown












Taylor Brown, Summer 2014, Blog Post 3

BLOG 3:
Hey everyone!

My summer is starting to wind down now; I’ve already presented my research to the Jacks Lab and and Dr. Jacks, but I still get two more weeks to do research!. Oh, and remember that lab outing I told you about? Yeah…that happened hahah. And it was so much fun! Still sore from running around Boston though haha. Someone calculated the distance we ran, and it was about 8.1 miles!



Duck (Slut) Dynasty…I was the Duck lol. We got lots of random pictures with people on the street, as well as lots of honks from cars driving by. And here is an example of one of the clue sheets:


And other costumes from lab members:


A duck, a playboy bunny, and a Broadie.




And here’s my poster!! (still a work in progress). Wish me luck on my poster presentation on Thursday! :D


And I believe this is my last post for the summer. Thanks for reading! It’s been a great summer, but boy am I homesick for LA! Anyway, Take care!


-Taylor(: